New tomb raider game11/13/2023 ![]() ![]() ![]() Fans can now sign up to "be the first to hear breaking news regarding the Tomb Raider franchise" via a newsletter. RELATED: Tomb Raider: Anniversary Fan Finds Special Copy of the Game at Secondhand StoreĪ recent update on the official Tomb Raiderwebsite has spurred rumors that an announcement may be imminent. The franchise has also been rebooted twice first in 2006 with Tomb Raider: Legend after the poor reception of 2003's Tomb Raider: The Angel of Darkness, then in 2013 after publisher Eidos Interactive was acquired by Square Enix.Īs of writing, almost nothing is known about the future of the Tomb Raider franchise which has also not had a mainline release since 2018's Shadow of the Tomb Raider, the final game in what is known as "The Survivor Trilogy." Speaking at the 2021 community update for the 25 Year Celebration, game director Will Kurzlik spoke about "working to unify these timelines," referring to the different Tomb Raider continuities that have been established by Core Design, the original developer, and the more recent Survivor Trilogy that has been helmed by Crystal Dynamics. The original Tomb Raider introduced one of the most iconic female protagonists in all of gaming and has been the subject of several sequels and spin-offs. The storied franchise has a long history with the first game releasing on the Sega Saturn, the original Sony PlayStation, and Microsoft's MS-DOS way back in 1996. ![]() While there are rumors about the upcoming Tomb Raider game, it is important to take them with caution and wait for official announcements.Ī small detail has sparked rumors that there is news about a new Tomb Raidergame on the horizon.Fans are eagerly awaiting news about the future of the Tomb Raider franchise, especially after the announcement of Lara Croft joining Call of Duty: Warzone.A recent update on the official Tomb Raider website has fueled speculation of an imminent announcement of a new game in the franchise. ![]()
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Minion halloween pumpkin template11/13/2023 ![]() These stories have been covered by the news, discussed at water coolers, and turned into Halloween costumes, but now they're taking on a new form.one that's rather round, orange, and full of seeds: we've turned them into printable pumpkin patterns! If you're tired of carving witches and bats, and aren't afraid to get the neighbors talking, then try your hand at any one of our 15 free printbale pop culture pumpkin patterns!Ĭarving Key: as with any standard pumpkin pattern, black areas should be cut out and removed completely, grey areas should be shaded, and white areas should remain uncarved. If you’re a purist when it comes to Halloween, Better Homes and Gardens offers quite a few free stencils on their website including bats, black cats, ghosts and traditional jack o’lantern faces.What an eventful year 2015 has been so far! The supreme court ruled to legalize same-sex marriage, a clumsy shark with bad rhythm stole the show at the Super Bowl, The Muppets got their own reality TV show, and Kanye West announced his intentions to run for P.O.T.U.S. Great for those texting tweens & teens □Ĭarve some of your favorite emojis into your pumpkin this year - find emojis for a thumbs up, pizza, puppy, ghost and many other fun ‘show your feelings’ stencils at Better Homes & Gardens. If you have teens who are fans of The Game of Thrones (the series, books or games), they love these Game of Thrones pumpkin stencils at Free Stencil Gallery!Īnd Pumpkin Glow has a great pattern for Gandolf the Wizard! SheKnows has an excellent round-up of patterns for Frozen, The LEGO movie and other film characters.Īny Jedi fan would love a Yoda pumpkin! You can find carving patterns for all your favorite Star Wars characters at. My daughter is all about Disney villians each year a new movie comes out & my whole family loves all the old & new characters from the Star Wars movies. One of the MOST popular ideas each year is carving pumpkins based on recently released films. Pumpkin Carving: Favorite Movie Characters ![]() If you’re a fan of classic cartoons, Cartoon Jr. personalities can be turned into jack o’lanterns - find Paw Patrol, Dora & Diego, Peppa Pig and more at the Nick Jr. Looking for Ironman, Ant Man, Thor, Captain America & even Groot? Visit Pumpkin Pile for lots of superhero stencils! Ironman, Thor & More Superhero Pumpkin Stencils And one of our BEST tips - cut a hole in the BOTTOM of your pumpkin so you can just sit it over the top of the candle!! It’s easier to light the candle that way and you don’t have an issue with a cut-off top that shrinks and falls into your jack o’lantern □Īnd if you’re looking for some FUN ideas for celebrating Halloween at Home this year, check out the 40+ Printable Halloween Games and Activities too!.If a section of the pumpkin falls off – don’t fret! You can use toothpicks to secure the piece back into place!.Carve out the smaller areas of your picture first and then the larger ones.Use a Pumpkin Carving Kit for quick & easy carving – we like the ones that have small pokers and mini-saws that are easy for kids to hold and use!.Keep a large bowl on the table to collect seeds (for toasting!). ![]() Be sure to tape down the paper stencil while tracing the design onto your pumpkin – we use a heavy duty clear packing tape to secure out patter.Cover the table with newspaper to make clean-up easier.We know first hand that it can sometimes take longer to FIND the right stencil than actually carve the pumpkin □ So browse this list of favorites for big kids & teens!Ī few tips for hosting your own carving session: ![]() Last year, we looked for more than an hour to find a really great Ironman stencil. Our kids love to find pumpkin stencils for their favorite book or movie characters each year so we’ve rounded-up a ton of favorites for you! We have a few Must-do’s Before Halloween Night at our house and one of them is carving pumpkins! (and making pumpkin seeds to snack on <– my kids made me type that □ ![]() Our last 100+ FREE Pumpkin Carving Stencils was so popular, I thought we should share some *NEW* ideas so you can quickly find the cool patterns you’ll need to carve the most awesome pumpkin ever!ĥ5 Free Pumpkin Carving Stencils & Patterns Find your favorite pumpkin carving stencils and patterns & create pumpkins of Disney favorites, Star Wars, IronMan & Superheros, the LEGO movie or even Game of Thrones! ![]() Logcat android studio11/13/2023 ![]() Get more information on the Android vitals > Overview page in Monitor your app's technical performance with Android vitals. This metric is one of the most important technical metrics and affects the discoverability of your app on Google Play. Tip: User-perceived crash rate is a core vital. ![]() To improve your ANR rate, fix ANR clusters with the highest number of affected users first.įor guidance on diagnosing and fixing ANRs, see Android Developers site. To see user-perceived ANR clusters, use the Issue visibility filter, and select only the issues that happen in the foreground. ANRs are grouped into clusters to help you identify when multiple ANRs may share the same root cause. This page shows the ANRs that contribute to your ANR rate metrics. ANRs on devices on Android 10 and below are available only in Play Console. When these dialogs appear, they're known as "application not responding" errors (or ANRs). If your app stops responding, users get a dialog that allows them to wait or close the app. Visit Google Play SDK Index to learn more about the SDKs you are using, and to make more informed SDK choices. Help us understand if the note was helpful or not by clicking the thumbs-up or thumbs-down icon at the bottom of the page. Following is the description of some important options highlighted in above image: Device selection. The following Logcat pane will appear on bottom of Android Studio. By clicking the Logcat Tool Button from the bottom of Android Studio. By clicking the main menu bar View > Tool Windows > Logcat. You will receive a Play Console Inbox message whenever a new note is added for a crash that affects your app. How to open Logcat By keyboard shortcut alt + 6. Look for "SDK provider notes" from the SDK provider on the Android vitals overview page ( Quality > Android vitals > Overview), which can help you understand the root cause of the issue, or how it can be prevented. ![]() SDK providers can also give guidance to app developers about crashes involving their SDK. SDK providers can see crashes you share in Google Play SDK Console. The crash report will include your app name, full stack trace, and other crash information. If a crash is marked as "possibly SDK-related" on the Android vitals overview page ( Quality > Android vitals > Overview), and you believe the SDK code is causing the crash, you can share it with the SDK provider to expedite crash resolution. When this happens, it’s often difficult to debug and resolve the issue as it occurs in code you don’t control. Occasionally, SDKs can cause technical issues in apps. To improve your crash rate, fix crash clusters with the highest number of affected users first.įor guidance on diagnosing and fixing crashes, see Android Developers site. The Logcat window in Android Studio acts as a central hub for displaying system and custom messages of applications across Android devices connected via the. To see user-perceived crash clusters, use the Issue visibility filter and select only the issues that happen in the foreground. One method is to use the Log Level dropdown menu, to filter based on log level, open this dropdown and select anything other than the default Verbose option. Crashes are grouped into clusters to help you identify when multiple crashes may share the same root cause. To get the most out of your application, Logcat has several levels of log messages, so Android Studio provides multiple ways of filtering the logcat output. This page shows the crashes that contribute to your crash rate metrics. To help you troubleshoot, the section includes device information, detailed crash information, the Logcat from the device, and a video of the error. ![]() ![]() Note: After you select a cluster, a separate section is displayed if an issue was found while testing your app to generate a pre-launch report. For additional details about a crash or an ANR error, select View details ( ) next to the item. Near the center of your screen, use the filters to help you find and diagnose issues.On the left menu, select Quality > Android vitals > Crashes and ANRs.Data comes from Android devices whose users have opted in to automatically share their usage and diagnostics data. Package Play Console, you can view data for crashes and "application not responding" (ANR) errors for your apps. ![]() Restart remote computer11/13/2023 ![]() Method 1: Using the Windows Remote Shutdown Command
Nidhogg bird music11/13/2023 ![]() Masego)," "Birds of Paradise," "Black Classical Music (feat. James Blake - Playing Robots Into Heavenįeatured Songs: "Big Hammer," "If You Can Hear Me," "Fall Back," "Fire The Editor," "I Want You to Know"įeatured Songs: "Afro Cubanism," "Marching Band (feat.Featured Albums:įeatured Songs: "get him back!" "all-american bitch," "ballad of a homeschooled girl," "the grudge"įeatured Songs: "Come Around and Love Me," "It Takes a Fool," "Give Me Another Day"įeatured Songs: "Mother," "Genesis," "Black Hole," "The King" WBEZ and Vocalo Radio's Ayana Contreras joins critic and reporter Cyrena Touros, Radio Milwaukee's Tarik Moody and host Robin Hilton as they share their picks for the best releases out on Sept. We've also got surreal dance-floor beats from James Blake, a staggeringly ambitious work from the singer Anjimile, Black Classical Music from Yussef Dayes and more. 8: Apollo Brown & Planet Asia - Sardines Ashley McBryde - The Devil I Know Blind Boys of Alabama - Echoes Of The South The Chemical Brothers - For That Beautiful Feeling Courtney Barnett - End of the Day Gaika - Drift Irreversible Entanglements - Protect Your Light Laufey - Bewitched Róisín Murphy - Hit Parade Teezo Touchdown - How Do You Sleep At Night? Tinashe - BB/ANG3L Tyler Childers - Rustin' in the Rain V - Layover ZZ Ward - Dirty Shine Leon Thomas & Barbara Hicks)" Lightning Round: Allison Russell - The Returner Romy - Mid Air Sparklehorse - Bird Machine Sylvester - Private Recordings, August 1970 Other notable releases for Sept. Venna & Charlie Stacey)," "Early Dayes," "Cowrie Charms (feat. Yussef Dayes - Black Classical Music Featured Songs: "Afro Cubanism," "Marching Band (feat. ![]() James Blake - Playing Robots Into Heaven Featured Songs: "Big Hammer," "If You Can Hear Me," "Fall Back," "Fire The Editor," "I Want You to Know" 5. Anjimile - The King Featured Songs: "Mother," "Genesis," "Black Hole," "The King" 4. Jalen Ngonda - Come Around and Love Me Featured Songs: "Come Around and Love Me," "It Takes a Fool," "Give Me Another Day" 3. Olivia Rodrigo - GUTS Featured Songs: "get him back!" "all-american bitch," "ballad of a homeschooled girl," "the grudge" 2. 8 : All Songs Considered The best albums out this week include Olivia Rodrigo's GUTS, James Blake's Playing Robots Into Heaven, Yussef Dayes' Black Classical Music and more. New Music Friday: The best releases out on Sept. ![]() Android studio for mac11/13/2023 ![]() For Android Emulator downloads, see the Emulator download archives. However, we recommend that you download the latest stable version or the latest preview version. The problem is I can't find the Android SDK on my machine! I assume it's there because otherwise the program wouldn't compile and run through Android Studio? Perhaps that's a bad assumption? I'm new to Macs (I'm used to Windows) so I don't know the best way to search for the Android SDK. This page provides an archive of Android Studio releases. Make sure the Android SDK platform-tools/ directory is included in your PATH environment variable, then execute: If you don’t know which chip to select, just click on the Apple icon in the top left corner and click on. ![]() All I want to do now is be able to build the app and install it on device from the command line as opposed to Android Studio. Readers like you help support XDA Developers. If you don't have any devices configured, you need to either create an Android Virtual Device to use the Android Emulator or connect a physical device. In the target device menu, select the device that you want to run your app on. For Example, Nox Player, ARChon for Mac, Android Studio, Bluestacks 3, and so on. Select the version of Android Studio that’s right for your Mac: Android Studio Hedgehog 2023.1. Android Emulator for Mac allows you to run Android apps on your Mac. Essentials Modern Android Quickly bring your app to life with less code, using a modern declarative approach to UI, and. I am working on a MBP 2.I have installed Android Studio on my MacBook Air (OS Version 10.11 El Capitan) and have successfully written a small "hello, world" app and installed on device (Nexus 7) and ran on AVD. The latest Canary build of Android Studio has added preliminary support for Macs powered by the ARM-based Apple Silicon M1 chip. To build and run your app, follow these steps: In the toolbar, select your app from the run configurations menu. Get early access to the latest features and improvements in Android Studio. ![]() Is there anyone developing for Android on a Mac who was able to increase this basic performance of Android Studio? Is there any hidden trick, combination of JVM and options able to make this app usable? Ive downloaded the Android Studio for Mac package from this link multiple times today. This definitely does NOT work on rosetta 2 as of. However, if you are using android studio, i presume you would like to also run the android emulator. Applications/Android Studio.app/Contents/binĪnd modified these values in studio.vmoptions: -Xms512mĪctually I have only increased the allocated memory for the first two of them. Android studio works on Mac M1 arm under rosetta 2. Rich layout editor with support for theme editing. Expanded template support for Google Services and various device types. Lets set up Android Studio on an Apple Silicon machine Oh is it still a bit quirky Here I show the steps on M1 Pro MacBook. Build variants and multiple APK generation. But I want to focus on this basic functionality.Īfter a bit of research I ended up increasing its heap space. Built on IntelliJ IDEA Community Edition, the popular Java IDE by JetBrains. I have also other problems, related to incredible bugs. In this article, we will learn what is Android Studio, why we need Android Studio and the installation process of Java JDK for Windows 10 and the process of installing Android Studio for Windows, Mac, Linux. It is almost impossible to use the graphical layout editor because it is sloooow. If I right-click I wait for the equivalent of geological ages before something is displayed. If I delete characters, I never know which one I reached without waiting for a couple of seconds. The main problem is that very often but not always (I would say almost always) it is not able to keep the pace of my editing: when I edit text, characters appear on the screen between one and two seconds later. ![]() I am not referring to the Android emulator, which was doubling the development time, but to the source code editor itself. Install Android Studio Set up your Android device Set up the Android emulator Agree to Android Licenses macOS setup Additional macOS requirements Next step System requirements To install and run Flutter, your development environment must meet these minimum requirements: Operating Systems: macOS, version 10.14 (Mojave) or later. Currently Android Studio is simply unusable: it is too slow. chn Dowload Android Studio (for MAC), sau khi ti v m phn mm android studio lên và tin hành cài t. ![]() I am trying to develop some native Android apps using Android Studio (latest) on OS X Yosemite. Hôm nay mình s hng dn các bn cài t android studio cho MAC OS, khi cài t android studio chúng ta cng s có luôn b Android SDK. ![]() Helpndoc personal edition 6.4.111/13/2023 ![]() ![]() The only restrictions are that the Personal Edition can't be used for commercial or profit purposes and a discreet banner is added at the bottom of each topic. HelpNDoc is completely free without any limit in feature. Download the free version of HelpNDoc to easily create the most amazing books, help, manuals and documentations by generating CHM, HTML, PDF, Word, iPhone. ![]() The only restrictions are that the Personal Edition cant be used for commercial or profit purposes and a discreet. This displays the license details dialog, including your HelpNDoc edition, serial number and license validity. Click the Manage license button in the License details section. Click the Help tab to access license details. Freeware Vista / Win10 / Win7 / Win8 / WinXP. Launch HelpNDoc on the computer where it needs to be deactivated, and click the File menu. Need to change one picture ? Just update the library item and it will be propagated to all the topics using it! AfterDawn > Software downloads > Desktop > Text editors > HelpNDoc Personal Edition v4.9.0.111. HelpNDoc Personal Edition: Quickly create complete help files, manuals, documentations and eBooks. HelpNDoc's user interface has been carefully designed to be clear and effective: the various tools are grouped using the popular ribbon design first introduced by Microsoft Office: contextual elements such as picture or table editing operations will only appear when needed thus greatly simplifying the overall experience.Īll the necessary tools needed to create great documentations are built right in HelpNDoc: the table of contents editor, WYSIWYG topic editor, keywords editor and library are some of the features provided to ease-up the process of writing amazing documentations and are an integral part of the HelpNDoc environment.Īll the media elements such as pictures, videos, documents, HTML code snippets and variables are managed by the library: those media elements are reusable as much as needed throughout the documentation project. HelpNDoc provides all the tools necessary to write complete help files and documentations ? From the table of contents to the final documentation layout, everything is included in HelpNDoc. ![]() Bato spring resort rates 201611/12/2023 ![]() ![]() ![]() You can drop off by San Pablo City, Laguna or the intersection bound to Dolores, Quezon.įrom there, take a Tricycle to Bato Springs. If there’s one thing that sets Laguna apart from other Philippine tourist destinations, it is having proximity to a wide range of hot spring resorts. ![]() terminal (JAM, DLTB or JAC Liner) bound to Lucena, Quezon, the fare is less than 130 pesos for an air-conditioned bus. Entrance Fee 70.00 Locker Fee (Opted not to rent a cottage) 100.00/2 50.00 Miscellaneous Expenses for Snacks 200.00/2 100.00 Tricycle to Hi-way 150.00/2 75.00 Bus bound for Buendia 127.00 Total: 624. Photos by Dona Jovita Garden Resort and California Beach Pansol. Miscellaneous Expenses for Snacks – 200.00/2 – 100.00 Locker Fee (Opted not to rent a cottage) – 100.00/2 – 50.00 Tricycle going to Bato Spring – 150.00/2 – 75.00 I must say the setting is just perfect for people who want to get away from humid and chaotic metropolitan. It’s not a 5-star hotel and resort but its atmosphere purely embraces the sense of nature. Reservations: First come, first served basis on huts and tables. The resort is designed to be Eco-friendly and sustainable. w/ 1 bed for two P 1,200 & up family room 2,500 & up Check in time Check out time 8:00am 4:00pm (For overnight) 9:00am 8:00am-next day 5:00pm 4:00pm-next day For more details about the rates you can check Bato Spring's website. The tricycles will send you directly to the resort for a fee of Php 150 per special trips. The resort is 5km from the main road and there are 5-seater tricycles piling up just where the bus will disembark you. The spring resort nestled on the foothills of Mt. Bato Springs, San Pablo City, Laguna, Philippines - May 15, 2016: people bathe in cold and clean rocky mountain spring water river on hot summer. Note that you have to inform the bus driver or attendant to drop you at the Maharlika Hi-way where there’s a Bato Springs signboard. Destination/ Product Rating Bato Springs Resort is located in San Pablo. If you’ll be taking the bus bound to Lucena from Pasay Terminal in Taft Ave., the fare is Php127 each passenger. Rates EDIT: ENTRANCE FEE (adults / kids). This pool has two water falls around it where you can sit and have your back massaged by the flowing water. The next day we ordered pancit for breakfast and take a final swim until we get back with our busy lives in Manila. This was the tiled swimming pool with impressive man-made falls at a corner.The main pool is about 4 feet deep and has a groto/cave hidden with a stream of falling water, water flows from this to a second pool which is about 2 feet deep. Fare: 140.00 Cubao to San Pablo (Bus, JAC Liner)ĥ0.00 San Pablo to Bato Springs Resort (Jeep)Ĩ5.00 Resort to Magallanes (hired the same jeepney, total fare was divided into 22) Things I did enjoy during our Overnight stay: Food (all meals are covered) & Cottage : Php 300.00 (depends on the pax) This was actually a get-together/team-building trip of some teams in our company, and I just happened to have a kind friend who cordially invited me and shouldered all the estimated damage, which by the way is summarized as follows: San Cristobal, San Pablo City, Laguna and is situated near the two majestic mountains, Mt. Time to log off for a while, breathe and slow down.įor thirsty wanderers like me, Bato Springs is like an oasis in the middle of the desert, and a perfect paradise that concretizes the fantasies that seemed so distant. Bato Springs See all things to do Bato Springs 3.5 67 reviews 5 of 17 things to do in San Pablo City Water Parks Open now 12:00 AM - 11:59 PM Visit website Call Email Write a review About Suggested duration More than 3 hours Suggest edits to improve what we show. Chaotic and busy urban life, pollution brought forth by the so-called “progress”, red-flagged emails, deadlines, alarms… stop. ![]() Downloading macos ventura stuck11/12/2023 ![]() This can depend on where you are in the installation process, but once it has started installing, I wouldn’t risk rebooting. It may make things worse – or possibly, much worse. It really isn’t advisable to reboot your Mac in the middle of an installation. ![]() You can try pressing Command– L to get more detailed information about the installation. However, they are often inaccurate and do not show enough detail to tell you whether the process is actually progressing or not. ![]() This is why it is often best to update your machine overnight on a weekend.Īlso, progress bars are supposed to make you feel better so you can see that the process is working. From start to finish, the update process could take the better part of a day to complete however, most updates will not take nearly this long. If it hasn’t been several hours yet, you should just wait and see if it will finish. The whole process can take anywhere from minutes to hours.
Klenow fragment extension11/12/2023 ![]() ![]() Microcon 30 (Amicon) cartridge purification: Pipet off the silicone oil. This protocol should generate 1000-1500 nmoles of completely double-stranded DNA template (the remaining 200-700 pmoles is a ladder of partially extended products ).ģ. Do not heat inactivate, as this accelerates the 3’ > 5’ exonuclease activity. Centrifuge briefly and stop the reaction by adding 12 µL of 100 mM EDTA. Gently mix the reaction and incubate at 37Â☌ for 30 min. ![]() If required for analytical purposes, add 1 µL of dCTP (specific activity 800 Ci/mmol) to the reaction or use an end-labeled primer. Mix the components in the order listed in a microfuge tube on ice: 90 µL of annealed template/primer, 30 µL of 10x Klenow Fragment buffer, 7 µL of 1 M MgCl2, 34 µL of 25 mM dNTPs (final concentration of 0.85 mM dNTP for a 100 nuc oligo), 45 units of Klenow Fragment and Type I water to a final volume of 300 µL. The annealed template and primer mixture from the previous step is used directly to set up the Klenow reaction. Fast cooling results in 10% more full-length extension product than slow annealing at 2Â☌/min in a thermal cycler (data not shown, but this is a consistent result).Ģ. Heat the mixture at 95Â☌ for 5 min, place the tube on ice for 5 min, then spin the contents briefly. Add 25 uL of silicone oil to prevent condensation effects. The mixture is vortexed and centrifuged briefly. In a siliconized microfuge tube, mix 9 µL of 10x annealing buffer with 2 nmol of gel-purified 5’ primer and 1.7 nmol of gel-purified template (a 1.2:1 ratio of primer:template) and adjust the volume to 90 µL with water. The primer is annealed to the synthetic DNA template prior to Klenow extension. Silicone oil (density 0.963, boiling point > 140Â☌) is available from Aldrich (Milwaukee, WI) as catalog # 14, 615-3.ġ. Microcon 30 cartridges are available from Amicon (Beverly, MA).ħ. Alternatively, the 5’ primer can be kinased with ATP (specific activity 3000 Ci/mmol, GE Healthcare ReadiVue), according to the protocol below.Ħ. Optional: dCTP (specific activity 3000 Ci/mmol, NEN, Boston, MA) can be used to body-label analytical reactions. ![]() Klenow Fragment is available from Roche Molecular (Indianapolis, IN) and other suppliers.ĥ. Discard if precipitated.ģ.Ē5 mM dNTPs: Combine 250 µL each of 100 mM dATP, dCTP, dGTP and TTP. Mix 5 mL of 1 M Tris-HCl, pH 7.4, 1 mL of 1 M MgCl2, 10 µL of 1 M DTT and 500 µL of 10 mg/mL BSA with 3.5 mL of Type I water. Store at -20Â☌.Ģ.đ0x Klenow buffer: 500 mM Tris-HCl, pH 7.4, 100 mM MgCl2, 1 mM dithiothreitol (DTT) and 500 µg/mL Bovine Serum Albumin (BSA). Mix 100 µL of 1 M Tris-HCl, pH 8.0 and 100 µL of 1 M MgCl2 with 800 µL Type I water. Klenow Fragment Extension (1 nmole scale)ġ.đ0x Annealing Buffer: 100 mM Tris-HCl, pH 8.0 and 100 mM MgCl2. ![]() AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |